Stul RFLP in the human ABL locus

StuI RFLP in the human ABL locus.

Source/Description: A 3.6 kb EcoRI fragment (lambda ABL3) containing portions of exons 7 and 8 and the intervening intron of the ABL oncogene was isolated from a chromosome 9-specific library (ATCC # LA09NS01) using a 1.7 kb Abelson murine leukemia virus probe (v-abl, ONCOR, Inc.). A single copy 1.8 kb BglII fragment (ABL3) was derived from the original lambda clone and detects the StuI RFLP. P...

Asp700 RFLP at the D8S45 locus.

Source/Description of Clone: Clone pALP13-8 is a single copy 750 bp EcoRV fragment, 0.9 kb 3' of exon 4, subcloned from a 6.6 kb BamHI fragment containing the complete human c-fos oncogene (FOS) (1). Polymorphisms: are detected with TaqI (A alleles) and BstNI (B alleles) (see Figure). A) TaqI: Presence of a 1.6 kb fragment or its allele, a 0.6 kb fragment. B) BstNI: Presence of an 0.85 kb fragm...

NcoI RFLP in the pseudogene (CYP2D8P) of the human debrisoquine 4-hydroxylase locus.

A notI RFLP in the human alpha 2-C4 adrenergic receptor locus (ADRA2RL2) detected by PFGE.

Description: The thiolester site of the human alpha-2-macroglobulin (A2M) is essential for the function of A2M as a proteinase inhibitor (1, 2). This site, which is encoded for by exon 24 of the A2M gene, was amplified by polymerase chain reactio (PCRa usinr tho primer 5'-CTGAAGAATTATCCCTGAAAC-3' and 5'-TGGTTCCTGCCATATCGCTC-3'. PCRs were performed in 100 /xl volumes containing 0.5 ng genomic DN...

A high-frequency RFLP at the human TFE3 locus on the X chromosome.

A polymorphism of D13S86 from the region 13q14.1 to 13q22 N.P.McHaffie-, R.Houwen,' + , W.Cavenee and D.W.Cox. 2 * 1 Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, department of Medical Genetics, University of Toronto, Toronto and Ludwig Institute for Cancer Research, Montreal, Canada A high-frequency RFLP at the human TFE3 locus on the X ch...